Sumon Pratihar, Eric T. Kool and his team from Stanford University just published new paper in 𝘈𝘯𝘨𝘦𝘸𝘢𝘯𝘥𝘵𝘦 𝘊𝘩𝘦𝘮𝘪𝘦, unlocking a new way of selective RNA modification: “𝘚𝘦𝘲𝘶𝘦𝘯𝘤𝘦-𝘚𝘱𝘦𝘤𝘪𝘧𝘪𝘤 𝘐𝘯𝘴𝘵𝘢𝘭𝘭𝘢𝘵𝘪𝘰𝘯 𝘰𝘧 𝘈𝘳𝘺𝘭 𝘎𝘳𝘰𝘶𝘱𝘴 𝘪𝘯 𝘙𝘕𝘈 𝘷𝘪𝘢 𝘋𝘕𝘈-𝘊𝘢𝘵𝘢𝘭𝘺𝘴𝘵 𝘊𝘰𝘯𝘫𝘶𝘨𝘢𝘵𝘦𝘴” Key innovationDNA-directed approach that utilizes a DNA oligonucleotide carrying a nucleophilic amine to promote site-selective SNAr reaction at RNA…
Then I would like to invite you to read our paper in RSC Chem. Biol. which also represents my first corresponding authorship: “𝘋𝘪𝘴𝘤𝘰𝘷𝘦𝘳𝘺 𝘰𝘧 𝘢𝘯 𝘦𝘹𝘲𝘶𝘪𝘴𝘪𝘵𝘦𝘭𝘺 𝘴𝘦𝘭𝘦𝘤𝘵𝘪𝘷𝘦 𝘞𝘋𝘙5 𝘤𝘩𝘦𝘮𝘪𝘤𝘢𝘭 𝘱𝘳𝘰𝘣𝘦 𝘢𝘤𝘤𝘦𝘭𝘦𝘳𝘢𝘵𝘦𝘥 𝘣𝘺 𝘢 𝘩𝘪𝘨𝘩-𝘲𝘶𝘢𝘭𝘪𝘵𝘺 𝘋𝘌𝘓–𝘔𝘓 𝘏𝘪𝘵” (DEL-ML = DNA Encoded Library + Machine Learning) Highlights✅ We demonstrate power of DEL-ML and how it can accelerate…
DNA methyltransferases (MTases) can recognize specific DNA sequences and transfer a methyl group from the S-adenosyl-L-methionine (SAM) cofactor to a target nucleobase. This ability has been exploited for sequence-specific DNA labeling using synthetic SAM analogues (SMILing, mTAG). However, both approaches (SMILing and mTAG) share following limitations:🔸 The SAM analogues are chemically reactive (labile aziridine rings…