While transition metals drive powerful bond-forming reactions in synthetic chemistry, their use for covalent modification of biomolecules has been largely underexplored and restricted to isolated proteins in vitro. Aryl-metal reagents for protein S-arylation have so far been dominated by palladium and gold complexes, but these systems require sterically bulky phosphine ligands that promote reductive elimination,…
E-STUB: A Precision Tool for Mapping E3 Ligase Substrates In march 2025, colleagues from Dana Faber and The Broad Institute published in ๐๐ข๐ต๐ถ๐ณ๐ฆ ๐๐ฉ๐ฆ๐ฎ๐ช๐ค๐ข๐ญ ๐๐ช๐ฐ๐ญ๐ฐ๐จ๐บ: “๐๐ฃ๐ช๐ฒ๐ถ๐ช๐ต๐ช๐ฏ-๐ด๐ฑ๐ฆ๐ค๐ช๐ง๐ช๐ค ๐ฑ๐ณ๐ฐ๐น๐ช๐ฎ๐ช๐ต๐บ ๐ญ๐ข๐ฃ๐ฆ๐ญ๐ช๐ฏ๐จ ๐ง๐ฐ๐ณ ๐ต๐ฉ๐ฆ ๐ช๐ฅ๐ฆ๐ฏ๐ต๐ช๐ง๐ช๐ค๐ข๐ต๐ช๐ฐ๐ฏ ๐ฐ๐ง ๐3 ๐ญ๐ช๐จ๐ข๐ด๐ฆ ๐ด๐ถ๐ฃ๐ด๐ต๐ณ๐ข๐ต๐ฆ๐ด” How it worksE-STUB (E3-Substrate Tagging by Ubiquitin Biotinylation) combines the principles of proximity labeling with the functional specificity of ubiquitin…
For decades, creating proteins with extended backbones (ฮฒ, ฮณ, ฮด-amino acids) inside living cells has been challenging. Researchers at University of California, Berkeley (Alanna Schepartz, Leah Roe), Yale University, Amgen and collaborators developedย BEAR reactionsย to address this challenge. ๐๐ก๐ฒ ๐๐จ๐๐ฌ ๐ญ๐ก๐ข๐ฌ ๐ฆ๐๐ญ๐ญ๐๐ซ?โ Extension of backbone can lead to improved phys-chem propertiesโ Better stability and resistance to…